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bio rad molecular imager chemidoc xrs system  (Bio-Rad)


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    Bio-Rad bio rad molecular imager chemidoc xrs system
    Bio Rad Molecular Imager Chemidoc Xrs System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 34936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/molecular+imager+chemidoc+system/ChemiDoc+Imaging+System/pmc13096964-369-20-26
    Average 99 stars, based on 34936 article reviews
    bio rad molecular imager chemidoc xrs system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Software:

    Article Title: Fusion protein-driven IGF-IR/PI3K/AKT signals deregulate Hippo pathway promoting oncogenic cooperation of YAP1 and FUS-DDIT3 in myxoid liposarcoma
    Article Snippet: .. Chemiluminescent signals were detected by the Molecular Imager ChemiDoc System (Image Lab Software; BioRad Laboratories). .. Densitometric quantification of the immunoblots (biological replicates) was performed using Image Lab software version 6.1 (Bio-Rad Laboratories).

    Article Title: SS18::SSX and BRD9 Modulate Synovial Sarcoma Differentiation
    Article Snippet: After blocking with 5% milk powder in TBS-T, incubation with the following primary antibodies was performed overnight (4 °C): SS18::SSX (Cell Signaling Technology, #72364), BRD9 (Cell Signaling Technology, #58906), BRD7 (Cell Signaling Technology, #15125), PBRM1/BAF180 (Cell Signaling Technology, 91894), ARID1A (Cell Signaling Technology, #12354), ARID1B (Cell Signaling Technology, #92964), E-Cadherin (Cell Signaling Technology, #3195), Snail (Cell Signaling Technology, #3879), Slug (Cell Signaling Technology, #9585), GAPDH (Cell Signaling Technology, #5174) and β-actin (Sigma-Aldrich, St. Louis, MO, USA, #A5441). .. Incubation with horseradish peroxidase-linked secondary antibodies was performed at room temperature for 1 h. Signal detection was performed with the SignalFire ECL and Elite Reagent chemiluminescence detection kit (Cell Signaling Technology) and the Molecular Imager ChemiDoc system (Image Lab Software, version 5.1; Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: A bipartite bacterial virulence factor targets the complement system and neutrophil activation.
    Article Snippet: The complement system and neutrophils constitute the two main pillars of the host innate immune defense against infection by bacterial pathogens.. Here, we identify T-Mac, a novel virulence factor of the periodontal pathogen Treponema denticola that allows bacteria to evade both defense systems.. We show that T-Mac is expressed as a pre-protein that is cleaved into two functional units.

    Article Title: SS18::SSX and BRD9 Modulate Synovial Sarcoma Differentiation.
    Article Snippet: After blocking with 5% milk powder in TBS-T, incubation with the following primary antibodies was performed overnight (4 ◦C): SS18::SSX (Cell Signaling Technology, #72364), BRD9 (Cell Signaling Technology, #58906), BRD7 (Cell Signaling Technology, #15125), PBRM1/BAF180 (Cell Signaling Technology, 91894), ARID1A (Cell Signaling Technology, #12354), ARID1B (Cell Signaling Technology, #92964), E-Cadherin (Cell Signaling Technology, #3195), Snail (Cell Signaling Technology, #3879), Slug (Cell Signaling Technology, #9585), GAPDH (Cell Signaling Technology, #5174) and β-actin (Sigma-Aldrich, St. Louis, MO, USA, #A5441). .. Incubation with horseradish peroxidase-linked secondary antibodies was performed at room temperature for 1 h. Signal detection was performed with the SignalFire ECL and Elite Reagent chemiluminescence detection kit (Cell Signaling Technology) and the Molecular Imager ChemiDoc system (Image Lab Software, version 5.1; Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: SS18-SSX drives CREB activation in synovial sarcoma
    Article Snippet: Next, protein extraction and phospho-kinase immunoblotting combining spotted antibodies for 46 kinase phosphorylation sites were performed as indicated by the manufacturer (Human Phospho-Kinase Array Kit, #ARY003, R&D Systems). .. Immunoblot development was performed using a chemiluminescence detection kit (ECL Amersham) and a Molecular Imager ChemiDoc system (Image Lab Software; Bio-Rad). ..

    Article Title: SS18-SSX drives CREB activation in synovial sarcoma
    Article Snippet: The SS18-SSX fusion protein was visualized using an anti-SS18/SYT antibody (#8819 Santa Cruz Biotechnology) detecting the N-terminus of SS18 (which is retained in the SS18-SSX fusion oncoprotein). .. Secondary antibody labeling (Bio-Rad) and immunoblot development were performed using an enhanced chemiluminescence detection kit (SignalFire ECL Reagent; Cell Signaling Technology) and a Molecular Imager ChemiDoc system (Image Lab Software; Bio-Rad), as described previously [ , ]. .. A set of pre-validated Stealth siRNAs for CREB , #2 = HSS102264, #3 = HSS175171; for IGF-IR : #4 = siRNA ID #110,754, #5 = siRNA ID #74 and a non-targeting negative control siRNA (BLOCK-iT Alexa Fluor Red Fluorescent Control) were purchased from Life Technologies.

    Article Title: A bipartite bacterial virulence factor targets the complement system and neutrophil activation
    Article Snippet: The following antibodies were purchased, including anti-His-tag (Invitrogen, Waltham, MA), anti-FLAG-tag (Sigma-Aldrich), anti-CXCR1 (Santa Cruz Biotechnology, Dallas, TX), anti-CXCR2 (Santa Cruz Biotechnology), anti-FPR1 (Invitrogen), anti-C5aR (Abcam, Cambridge, United Kingdom), and anti-CD11b (Abcam). .. Immunoblots were developed using a horseradish peroxidase-conjugated secondary antibody with enhanced chemiluminescence (ECL) assays; signals were quantified using the Molecular Imager ChemiDoc system with the Image Lab software (Bio-Rad Laboratories, Hercules, CA), as previously described (Kurniyati et al, ). ..

    Article Title: Exploiting WEE1 kinase activity as FUS::DDIT3-dependent therapeutic vulnerability in myxoid liposarcoma
    Article Snippet: .. Targets were labelled with HRP-linked secondary antibodies and chemiluminescent signal detection was carried out with the Signalfire ECL Reagent and the Molecular Imager ChemiDoc System (Image Lab Software, version 5.1, Bio-Rad Laboratories). ..

    Incubation:

    Article Title: SS18::SSX and BRD9 Modulate Synovial Sarcoma Differentiation
    Article Snippet: After blocking with 5% milk powder in TBS-T, incubation with the following primary antibodies was performed overnight (4 °C): SS18::SSX (Cell Signaling Technology, #72364), BRD9 (Cell Signaling Technology, #58906), BRD7 (Cell Signaling Technology, #15125), PBRM1/BAF180 (Cell Signaling Technology, 91894), ARID1A (Cell Signaling Technology, #12354), ARID1B (Cell Signaling Technology, #92964), E-Cadherin (Cell Signaling Technology, #3195), Snail (Cell Signaling Technology, #3879), Slug (Cell Signaling Technology, #9585), GAPDH (Cell Signaling Technology, #5174) and β-actin (Sigma-Aldrich, St. Louis, MO, USA, #A5441). .. Incubation with horseradish peroxidase-linked secondary antibodies was performed at room temperature for 1 h. Signal detection was performed with the SignalFire ECL and Elite Reagent chemiluminescence detection kit (Cell Signaling Technology) and the Molecular Imager ChemiDoc system (Image Lab Software, version 5.1; Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: SS18::SSX and BRD9 Modulate Synovial Sarcoma Differentiation.
    Article Snippet: After blocking with 5% milk powder in TBS-T, incubation with the following primary antibodies was performed overnight (4 ◦C): SS18::SSX (Cell Signaling Technology, #72364), BRD9 (Cell Signaling Technology, #58906), BRD7 (Cell Signaling Technology, #15125), PBRM1/BAF180 (Cell Signaling Technology, 91894), ARID1A (Cell Signaling Technology, #12354), ARID1B (Cell Signaling Technology, #92964), E-Cadherin (Cell Signaling Technology, #3195), Snail (Cell Signaling Technology, #3879), Slug (Cell Signaling Technology, #9585), GAPDH (Cell Signaling Technology, #5174) and β-actin (Sigma-Aldrich, St. Louis, MO, USA, #A5441). .. Incubation with horseradish peroxidase-linked secondary antibodies was performed at room temperature for 1 h. Signal detection was performed with the SignalFire ECL and Elite Reagent chemiluminescence detection kit (Cell Signaling Technology) and the Molecular Imager ChemiDoc system (Image Lab Software, version 5.1; Bio-Rad Laboratories, Hercules, CA, USA). ..

    Western Blot:

    Article Title: A bipartite bacterial virulence factor targets the complement system and neutrophil activation.
    Article Snippet: The complement system and neutrophils constitute the two main pillars of the host innate immune defense against infection by bacterial pathogens.. Here, we identify T-Mac, a novel virulence factor of the periodontal pathogen Treponema denticola that allows bacteria to evade both defense systems.. We show that T-Mac is expressed as a pre-protein that is cleaved into two functional units.

    Article Title: SS18-SSX drives CREB activation in synovial sarcoma
    Article Snippet: Next, protein extraction and phospho-kinase immunoblotting combining spotted antibodies for 46 kinase phosphorylation sites were performed as indicated by the manufacturer (Human Phospho-Kinase Array Kit, #ARY003, R&D Systems). .. Immunoblot development was performed using a chemiluminescence detection kit (ECL Amersham) and a Molecular Imager ChemiDoc system (Image Lab Software; Bio-Rad). ..

    Article Title: SS18-SSX drives CREB activation in synovial sarcoma
    Article Snippet: The SS18-SSX fusion protein was visualized using an anti-SS18/SYT antibody (#8819 Santa Cruz Biotechnology) detecting the N-terminus of SS18 (which is retained in the SS18-SSX fusion oncoprotein). .. Secondary antibody labeling (Bio-Rad) and immunoblot development were performed using an enhanced chemiluminescence detection kit (SignalFire ECL Reagent; Cell Signaling Technology) and a Molecular Imager ChemiDoc system (Image Lab Software; Bio-Rad), as described previously [ , ]. .. A set of pre-validated Stealth siRNAs for CREB , #2 = HSS102264, #3 = HSS175171; for IGF-IR : #4 = siRNA ID #110,754, #5 = siRNA ID #74 and a non-targeting negative control siRNA (BLOCK-iT Alexa Fluor Red Fluorescent Control) were purchased from Life Technologies.

    Article Title: A bipartite bacterial virulence factor targets the complement system and neutrophil activation
    Article Snippet: The following antibodies were purchased, including anti-His-tag (Invitrogen, Waltham, MA), anti-FLAG-tag (Sigma-Aldrich), anti-CXCR1 (Santa Cruz Biotechnology, Dallas, TX), anti-CXCR2 (Santa Cruz Biotechnology), anti-FPR1 (Invitrogen), anti-C5aR (Abcam, Cambridge, United Kingdom), and anti-CD11b (Abcam). .. Immunoblots were developed using a horseradish peroxidase-conjugated secondary antibody with enhanced chemiluminescence (ECL) assays; signals were quantified using the Molecular Imager ChemiDoc system with the Image Lab software (Bio-Rad Laboratories, Hercules, CA), as previously described (Kurniyati et al, ). ..

    Antibody Labeling:

    Article Title: SS18-SSX drives CREB activation in synovial sarcoma
    Article Snippet: The SS18-SSX fusion protein was visualized using an anti-SS18/SYT antibody (#8819 Santa Cruz Biotechnology) detecting the N-terminus of SS18 (which is retained in the SS18-SSX fusion oncoprotein). .. Secondary antibody labeling (Bio-Rad) and immunoblot development were performed using an enhanced chemiluminescence detection kit (SignalFire ECL Reagent; Cell Signaling Technology) and a Molecular Imager ChemiDoc system (Image Lab Software; Bio-Rad), as described previously [ , ]. .. A set of pre-validated Stealth siRNAs for CREB , #2 = HSS102264, #3 = HSS175171; for IGF-IR : #4 = siRNA ID #110,754, #5 = siRNA ID #74 and a non-targeting negative control siRNA (BLOCK-iT Alexa Fluor Red Fluorescent Control) were purchased from Life Technologies.



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